University of California, Riverside

Plant Transformation Research Center



Training and Tours


Training and Tours

Training and tours are offered around the year.

Workshops and personal training offer:
  • Bacterial and plant transformation
  • Gene (cloning, genomic, PCR, qPCR, RT-PCR)
  • Imaging techniques for the detection of foreign genes
  • Molecular analysis of transgenic plants (Southern, Northern and Western blot analyses, and enzyme assays for reporter genes)
  • Direct gene transfer by particle bombardment
  • Establishment and growth of transgenic plants in biosafety level 2 (BL2) greenhouses.

UCR students, staff, faculty, and international visiting scholars comments/reviews

During the last years PTRC trained:

  • 46 students from UCR’s College of Natural and Agriculture Sciences CNAS (Botany and Plant Sciences, Biology, Bioengineering)
  • 3 high school students (Corona and Riverside unified school districts)
  • 9 international graduate students
  • 18 researchers from different institutions


Reema Saleh
Bioengineering
Undergraduate
2010-2014

I had an amazing experience working at PTRC, which allowed me to have a hands on experience that contributed to my comprehension of my studies. As a bioengineering student, I was able to apply in the laboratory what I learned in the classroom. Now I have gained four years of experience over my peers and counterparts. Most undergraduates do not have the same opportunity I have had, and now I will be able to graduate with a head start.


Carlos
F. Castillo
Graduate Student
Visiting Scholar
2012-2014

I had a wonderful learning experience at PTRC. When I first came here from Colombia, I had no prior knowledge about in vitro plant tissue culture and transformation. Now, I am very happy that I have learned so much. I am confident that I can now use what I have learned and apply it to my future work as well as to teach others. I was also fortunate enough to work with such an amazing and diverse group of people at my stay here at PTRC, which also enhanced my learning about many different cultures.

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Jose David Cortez, MSc
Visiting  Scholar
2013

I was  at the UCR Plant Transformation Center to be trained  in the use of different molecular biology and plant transformation techniques using Arabidopsis and rice as model plants. This wonderful experience allow me to apply these techniques in my future research  to study gene function.  Thanks to Dr. Orozco-Cárdenas and the PTRC’s staff.


Mariam Saleh
English
Undergraduate
2011-2014

Although my majors are both within the humanities fields, I began working at PTRC maintaining the lab, which later grew into becoming a lab assistant working on plant tissue culture and transformation. This was a wonderful opportunity for me to learn outside of my field of study and future aspirations of law practice, allowing me to gain a sense of professional research.


Gabriela
Rodriguez
Biology
Undergraduate
2012-2013

Working in the Plant Transformation Research Center allowed me to learn many new skills and  techniques. It also gave me the opportunity to apply knowledge learned in previous laboratory courses in a real-world setting. It was beneficial to work both independently and as a team with other undergraduate students to complete projects. I am thankful for the experience gained from working in the PTRC and it will help me in my future career endeavors. 

 

 


Deema 
Akari
Bioengineering
Undergraduate
2012-2013

My time since 2012 to 2013 at the Plant Transformation Research Center was filled with many valuable learning experiences that will always be significant to me. The Center and its staff taught me many skills in terms of lab protocols and the proper way to assess and address a problem or research question. While the work I do in the future may not always pertain to plant transformation, there were many aspects of the work I did during my time at the Center that have prepared me for any type of science or academia related work that I may hope to do in my coming career.

 


Neidi Chavez
Computer Science
Undergraduate 2010-2013

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When I first started working as an undergraduate at the Plant Transformation Research Center, I was a Biochemistry major.  After some time at school I changed to Computer Science.  However I continued working at PTRC because of the valuable lessons I learned there.  Some of the lessons I learned in time management and problem solving skills transferred well into the field of Computer Science.  Working in a lab environment made me a more careful planner and a better team player.  Even though my work in the future will probably not be in a lab, I'm grateful for the lessons and friends I made at the PTRC.

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Khalid Akari Bioengineering Undergraduate 2011 - 2013

The Plant Transformation Research Center (PTRC) has provided me with an amazing learning experience within a lab environment. I loved the family-like environment within the lab as it provided a comfortable learning setting. As a bioengineering student at the University of California at Riverside, I have learned certain concepts within my classes that I was able to apply with hands on experience within the lab. PTRC has provided me with wonderful work experience opportunities within my field, which I am able to put on my resume. Overall my experience at PTRC was a wonderful learning opportunity and a great opportunity to meet great people who will forever be enshrined in my memories. I could easily say that my experience at PTRC is one of the most wonderful experiences at the University of California at Riverside.

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Karina Tercero
Sociology
Undergraduate
2011-2013

Being Sociology major, I have always had a significant interest with the emphasis of statistical sociology and the association that it has with research. In addition, I have noticed the importance of having a structure that creates a foundation to begin a project within research. Therefore, having the opportunity to work at PTRC has been immensely gratifying.  Working inside of PTRC labs has allowed me to observe the entire process of what a successful research facility does. For example, very essential components to working at PTRC are planning, proper time management, and having the adequate materials and equipments such as greenhouses. Lastly, seeing how deeply dedicated the rest of the team at PTRC is in regards to plant genetic transformation techniques.

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Tomas Lagunas
Biology
Undergraduate
2009-2012 

Working at the PTRC for three years was an extraordinary encounter, where I had the privilege to interact with exceptional scientists that provided their guidance and skills towards my development as a scientist; consequently, the transition from academia to industry was natural since I had already obtained the necessary knowledge. 

 


Jennifer Shin
Biology
Undergraduate
2010-2012

During my two years at the Plant Transformation Research Center, I was trained in  in vitro plant micro-propagation and plant transformations techniques. This experience helped me gain skills which I have been able to apply in pharmacy school lab classes that  require these type of aseptic techniques. 


Lina Hoyos
Psychology
Undergraduate
2009-2011

 

During my time training at PTRC, I had the opportunity to work with an amazing team that guided me in different activities related to Genetic Engineering, highly increasing my desire to become part of academia. Although my current area of study lies within the Humanities, I feel very fortunate as the experience helped me build research and interpersonal skills that help in my professional life today


Kathya Jovel Ruiz
Riverside Community College
Undergraduate
2010-2011

In 2010-2011, I had the opportunity to conduct an internship at PTRC, under the supervision of Dr. Orozco-Cárdenas. I learned tissue culture of asparagus, Agrobacterium-mediated transformation of tomato cells, and how to handle experimental plants in a greenhouse. The working environment was terrific. Now, as a nursing student in Canada, I realize how important my time at PTRC was to build up my critical thinking and to be able to integrate productive work teams. I thank PTRC for such a valuable experience.


Dr. Lea Cordiki
Botany & Plant Sciences Researcher
2013

 

For the last six months, I have been collaborating trained in in vitro tissue culture techniques at PTRC by  Dr. Martha L.  Orozco-Cárdenasin a project related to the Conservation of California native plants that are difficult to propagate from seeds.  Our goal is to develop efficient protocols to optimize the tissue culture of rare and endangered  plant species for landscaping or ecological restoration


Sandra Pulido
Graduate Student Visiting Scholar
2011-2013

 I came to the PTRC as a graduate student for two years in order to complete my Ph.D. program. This was an incredible opportunity for me to enrich my knowledge and experience in plant transformation. I also learned and improved my research skills for planning and performing  experiments in the lab and in the greenhouse. During this time, I had an enjoyable experience of working in an international environment with high  scientific standards. In special, I am truly grateful to Dr. Martha  Orozco-Cárdenaswho led my  constant process of scientific learning with a high human quality. At the same time, I would like to thank the scientific staff of PTRC and the Botany and Plant Sciences Department, who helped in many different ways during my learning  process.


Dora J. García
Graduate Student
Visiting Scholar
2009 - 2013

 

PTRC was a great place for me to grow academically and personally. During my time at PTRC I had the chance to be trained  in in vitro plant tissue culture and plant genetic transformation. The philosophy of PTRC are ethics, respect, and good communicationhelp me to be more successful with my work. I would like to thank Dr. Orozco-Cárdenas and the PTRC staff for allowing me to endure this unique experience in research.


Walter R. Lopez
Graduate Student
Visiting Scholar
2009 - 2013

 

During my time at PTRC, I was trained in plant tissue culture, plant genetic transformation, and  molecular biology. Dr. Orozco and her team  gave me the opportunity to grow both academically and personally. I thank Dr. Orozco-Cárdenas and Dr. Narváez-Vásquez for giving me the opportunity to gain experience in molecular analysis of the transgenic crops. 


Nelson Ceballos
Graduate Student
Visiting scholar 2011

The training in plant biotechnology at UCR’s Plant Transformation Research Center was crucial to complete my PhD program. Once I came back to my country I transferred those experiences to my undergraduate and graduate students, and created different research groups for investigating different important aspects in our horticultural field. 


Fredy Betancur
Graduate Student
Visiting Scholar
2011
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I was trained in plant genetic transformation at PTRC under the supervision of Dr. Martha L. Orozco-Cárdenas. This training was very useful to enrich my knowledge in the area of biotechnology and to define my future career.  It was an unforgettable experience!


Alexander Rebolledo, PhD
Visiting Scholar
2010

 

During my internship at UCR, I had the opportunity to expand my knowledge in plant physiology and cell biology. I also envisioned the possibilities of applying molecular biology techniques in my research. This was a great opportunity to start a collaboration between researchers at UCR and my institution.


Ana C. Morillo
Graduate Student
Visiting Scholar
2008

During my  internship at PTRC, I was trained in plant cell and molecular biology techniques under the mentorship of Dr. Javier NarváezVasquéz, and  Dr. Martha L. Orozco-Cárdenas.This training helped me to  improve my analytical skills as well as good hands to do experiments with precision and accuracy. This internship was crucial in projection of the development of my professional career.

Yacenia Morillo
Graduate Student
Visiting Scholar 2008

During my time at the Plant Transformation Research Center, I was trained in plant  in vitro tissue culture and genetic transformation techniques under the direction of Dr. Martha L. Orozco Cárdenas.  I was also awarded a scholarship by UCR’s Extension Center to attend  English (ESL) courses, which helped me to improve my English proficiency. The human talent and the valuable knowledge imparted by PTRC's faculty and staff have had a great impact on my professional career and personal life.



Edwin Restrepo
Graduate Student Visiting Scholar 2007

I was  at the UCR Plant Transformation Center to be trained  in the use of different molecular biology and plant transformation techniques. This training helped me to  improve my  English and analytical skills in the laboratory. Thanks to Dr. Orozco-Cárdenas.


Aliona Glijin, PhD
Visiting Professor
2006

 

I was trained in different technologies for the production and the molecular analysis of transgenic plants at the PTRC under the directorship of Dr. Martha L. Orozco-Cárdenas. This fellowship program was granted by CRDF-MRDA. An international collaboration was established between the PTRC at UCR and the Moldova State University (MSU). The scientific results as product of these Fellowship training program are published in  eleven scientific papers and discussed in specialized international conferences. I am very grateful to Dr. Javier Narvaez and Dr. Orozco-Cárdenas. for their kind hospitality and scientific assistance. Thanks also to all members of the PTRC team.  All of them helped me not just to accumulate a great baggage of knowledge and experience in the field, but also showed me a very  interesting country, with a very rich culture.


Angela Port, PhD
Visiting Professor 2005

At the PTRC, I  participated in many activities such as genetic transformation of plant cells and tissues, and molecular analyses of transgenic plants for the presence of foreign genes. The PTRC helped me to develop a screening protocol for the detection of GMOs for the establishment of a GMO regulatory system in my country.

 

More Information 

General Campus Information

University of California, Riverside
900 University Ave.
Riverside, CA 92521
Tel: (951) 827-1012

Department Information

Plant Transformation Research Center
South Campus Dr. Head house no. 2
Riverside, CA 92521-0124

Tel: (951) 827-2885
Fax: (951) 827-6325
E-mail: mlorozco@citrus.ucr.edu

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