University of California, Riverside

Plant Transformation Research Center



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Curriculum Vitae

Martha Lucia Orozco-Cárdenas

Director, Plant Transformation Research Center
University of California, Riverside
South Campus Dr. Head House No. 2
Riverside, CA 92521-0124  

Phones: 
(951) 827-2885 (Lab)
(951) 827-6325 (Office)
mlorozco@ucr.edu
http://www.ptrc.ucr.edu             

Download CV-MLOC 3-18-14.doc 

martha

I. Education

  • BS. Agronomist engineer, Universidad Nacional de Colombia, Palmira, Valle, Colombia, South America. 1976-1981.
  • MSc. Washington State University, Pullman, WA -USA. 1991-1993.
  • PhD. Plant physiology Program, Washington State University, Pullman, WA, USA. 1997-2000.

II. Professional positions held

  • 2002 - present. Director, Plant Transformation Research Center, Department of Botany and Plant Sciences, University of California – Riverside.
  • 2000 - 2002. Post Doctoral Fellow, Dr. CA. Ryan Laboratory, Institute of Biological Chemistry, Washington State University, Pullman, WA, USA
  • 1997 - 2000. Research Assistant Fellow, PhD Plant Physiology Institute of Biological Chemistry, Washington State University Pullman, WA, USA
  • 1993 - 1997. Principal Investigator. Biotechnology National Research Program, (CORPOICA), Santa Fé de Bogotá, Colombia.
  • 1996 (May to September). Visiting Scientist. Boyce Thompson Institute for Plant Research Inc. Cornell University, Ithaca, NY, USA
  • 1995 (February to May). Visiting Scientist. Laboratory of Tropical Crop Improvement, Catholic University of Leuven, Belgium.
  • 1994 (November). Visiting Scientist. International Center for Genetic Engineering and Biotechnology – ICGBE, New Delhi, India.
  • 1991 - 1993. Research Assistant – Fellow. MSc. Horticulture, Institute of Biological Chemistry, Washington State University, Pullman, WA, USA.
  • 1988-1991. Research Assistant. Institute of Biological Chemistry, Washington State University, Pullman, WA, USA.
  • 1984 - 1987. Manager. Private Sugarcane Company, Cali-Colombia, SA.
  • 1981 - 1984. Rural extensionist. Corporacion Autonoma Regional del Cauca (CVC), Cali, Colombia, SA.
  • 1979 – 1981. Research Assistant. International Rice Program. International Center for Tropical Agriculture (CIAT). Cali, Colombia, SA.

III. Awards and Honors

  • 2013. UCR. Enviromental Health & Safety. Blue ribbon award for outstanding commitment to laboratory safety, 2012-2013.
  • 2012. UCR. Enviromental Health & Safety. Yellow ribbon award for outstanding commitment to laboratory safety, 2011-2012.
  • 2012. UNAD. Universidad Nacional. Award International conference on Agricultural Biotechnology held in Pereira-Colombia. August 24, 2012.
  • 2012. UCR. Chancellor’s Recognition of Staff and Faculty Program- Recognition of 10 years of achievement award.
  • 2009. National University of Colombia. Award National University of Colombia (Palmira-Colombia) - Resolution No. 071, Acta 017, November 6, 2009.
  • 2005. National Science Foundation, (NSF-USA) - Travel Fellowship – Third International Conference Ecological Chemistry, Moldova, May 16-21, 2005
  • 1999. Washington State University - Helen and Loyal H. Davis, Graduate Student Award. Washington State University, Pullman, WA, USA, 1999
  • 1997. UNESCO, Fellowship Program. Visiting Scientist. Boyce Thompson Institute for Plant research Inc. Cornell University, Ithaca, NY, 1997
  • 1996. USDA, Cochran Fellowship Program. Visiting Scientist. Boyce Thompson Institute for Plant research Inc. Cornell University, Ithaca, NY, 1996
  • 1995. EU-European Union. Fellowship Program. Visiting Scientist. Laboratory of Tropical Crop Improvement, Catholic University of Leuven, Belgium, 1995
  • 1994. COLCIENCIAS. Beneficiary of the Research Incentive Program, Instituto Colombiano para el desarrollo de la Ciencia y la Tecnologia, Santa fê de Bogota, Colombia, SA. 1995
  • 1994. UNESCO. Fellowship Program. Visiting Scientist. International Center for Genetic Engineering and Biotechnology – ICGBE, New Delhi, India, 1994
  • 1993. COLCIENCIAS. Beneficiary of the National Return Program of Colombian. Researchers, sponsored by the Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnologia – Santa fé de Bogotá, Colombia,SA. 1993.
  • 1981. UN-Colombia. Meritorious thesis award, Universidad Nacional de Colombia, Palmira Valle, Colombia, South America, 1981.

IV. Publications

A. Journal Articles
  1. Chetty V, Ceballos, N, Narváez-Vásquez, J, Garcia, D, Lopez, W, Orozco-
  2. Cárdenas, ML. (2013) Evaluation of four Agrobacterium tumefaciens strains for the genetic transformation of Tomato (Solanum lycopersicum L. cultivar Micro-Tom. Plant Cell Rep. 32: 239-247.
  3. Narváez-Vásquez, J, Orozco-Cárdenas, ML, Ryan, CA. (2007). Systemic wound signaling in tomato leaves is cooperatively regulated by multiple plant peptides. Plant Mol. Biol. 65:711-718.
  4. Orozco-Cárdenas, ML, Ryan, CA. (2003). Polygalacturonase ß-subunit antisense gene expression in tomato plants leads to a progressive enhanced wound response and necrosis in leaves and abscission of developing flowers. Plant Physiol. 133, 1-9.
  5. Orozco-Cárdenas, ML, Ryan, CA. (2002). Nitric oxide modulates wound signaling in tomato plants. Plant Physiol. 130, 487-493.
  6. CR. Pineda, N. Toro, Perea, J. Narvaez, ML. Orozco, A. Laignelet, and H. Cárdenas. (2002). Genetic transformation by Agrobacterium tumefaciencs of embryogenic cell suspension of plantain "Dominico Harton" (Musa AAB Simmonds). InfoMusa. 11, (2), 9-13.
  7. Orozco-Cárdenas, ML, Narváez-Vásquez, J, Ryan, CA. (2001). Hydrogen peroxide acts as a second messenger for the induction of defense genes in tomato plants in response to wounding, systemin, and methyl Jasmonate. Plant Cell 13, 1-14.
  8. Hernandez, J, Mariño, L, Orozco, ML, Narváez, J. (2000). Microscopic, biochemical and molecular characterization of native isolates of Bacillus thuringiensis from Colombia. Astrolabio (Colombia) 2, 13-18.
  9. Orozco-Cárdenas, ML, Ryan, CA. (1999). Hydrogen Peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway. Proc. Natl. Acad. Sci. USA 96, 6553-6557.
  10. Bergey, D.R., Orozco-Cárdenas, ML, De Moura, D and Ryan, CA. (1999). A wound- and systemin-inducible polygalacturonase in tomato leaves. Proc. Natl. Acad. Sci. USA 96, 1756-1760.
  11. Pineda, CR. and Orozco-Cárdenas, ML. (1996). Agrobacterium tumefaciens como el mejor ingeniero genetico de la naturaleza. Innovacion y Ciencia. Santa fé de Bogotá, Colombia. ACAC, 5, 26-32.
  12. Narváez-Vásquez, J, Pearce, G, Orozco-Cárdenas, ML, Franceschi, VR., Ryan, CA. (1995). Autoradiographic and biochemical evidence for the systemic translocation of systemin in tomato plants. Planta 195, 593-600.
  13. McGurl, B, Orozco-Cárdenas, ML, Pearce, G, Ryan, CA. (1994). Over-expression of the prosystemin gene in transgenic tomato plants generates a systemic signal that constitutively induces proteinase inhibitor synthesis. Proc. Natl. Acad. Sci. USA. 91, 9799-9802.
  14. Narváez-Vásquez, J, Orozco-Cárdenas, ML, Ryan, CA. (1994). A Sulfhydryl Reagent Modulates Systemic Signaling for Wound-Induced and Systemin-Induced Proteinase Inhibitor Synthesis. Plant Physiol. 105, 725-730.
  15. Orozco-Cárdenas, ML, McGurl, B., Ryan, CA (1993). Expression of an antisense prosystemin gene in tomato plants reduces resistance toward Manduca sexta larvae. Proc. Natl. Acad. Sci. USA 90, 8273-8276.
  16. McGurl, B, Pearce, G, Orozco-Cárdenas, ML, and Ryan, CA. (1992). Structure, expression, and antisense inhibition of the systemin precursor gene. Science, 225, 1570-1573.
  17. Narváez-Vásquez, J, Orozco-Cárdenas, ML, and Ryan, CA. (1992). Differential expression of a chimeric CaMV-tomato proteinase inhibitor I gene in transformed nighshade, tobacco and alfalfa plants. Plant Mol. Biol. 20, 1149-1157.
B. Chapters and Symposia Proceedings
  1. Orozco-Cárdenas, ML, Chetty, V, Narváez-Vásquez, J. (2013). Potato (Solanum tuberosum L.) Methods in Molecular biology. Third edition. Ed. K. Wang. Humana Press. Submitted: August 15, 2013.
  2. Orozco-Cárdenas, Martha L., Narváez-Vásquez, Javier, Dora Garcia. (2013). Tomato
  3. (Lycopersicum esculentum) transformation via Agrobabacterium transformation. Methods in Molecular biology. Third edition. Ed. K. Wang. Humana Press. Submitted: August 15, 2013.
  4. Orozco-Cárdenas, M L, Pearce, G, Narváez-Vásquez, J. (2012). Hydroxyproline-Rich Systemin Glycopeptides - Signaling Peptides for Plant Defense Localized in Cell Walls. Plant Peptide Signals. Ed. A. Huffaker. Wiley-Blackwell. Annual Plant Reviews. Submitted: February 15, 2012.
  5. Narváez-Vásquez, J, Orozco-Cárdenas, ML, Pearce, G. (2009). Plant peptide signals. In, Amino acids, Peptides and Proteins in Organic Chemistry. Ed. A.B. Hughes. Wiley-VCH publishers. Vol. 2, pp, 569-631.
  6. Narváez-Vásquez, J, Orozco-Cárdenas, ML. (2007) Systemin and AtPeps: Defense-related peptide signals. In, Induced Plant Resistance to Herbivory. Ed. Andreas Schaller, Springer, pp. 313-327.
  7. Narváez-Vásquez, J, Orozco-Cárdenas, ML, Ryan, CA. (1996). Expression of proteinase inhibitor genes in plants. In: Proc. Int. Worshop on Transgenic Tech. in plants, Bogota. Eds. O. Acosta and K.D. Webster, Universidad Nacional de Colombia and Scottish Crop Research Institute. pp. 36-42.
  8. Ryan, CA., McGurl, B, Pearce, G, Farmer, EE, Narváez-Vásquez, J, Orozco-Cárdenas, ML. (1994). Oligogalacturonides induce proteinase inhibitor synthesis via the octadecanoid pathway. In, Plant Molecular Biology Molecular Genetic Análisis of Plant Development and Metabolism, Eds. G. Coruzzi and P. Puigdomenech, Springer-Verlag, Berlin Heidelberg, pp. 229-233.
  9. Orozco-Cárdenas, ML, McGurl, B, Pearce, G, Narváez-Vásquez, J, Ryan, CA. (1993). Engineering a signaling pathway gene in a crop plant modulates resistance toward an insect pest. In, Proceedings of the International Workshop of the Phaseolus Beans Advance Research Network (BARN), Sept. 7-10, 1993, CIAT, Cali-Colombia, pp 168-174.
  10. Ryan, CA, Pearce, G, Johson, S, McGurl, B, Orozco-Cárdenas, ML, Farmer, EE, Ryan, CA. (1992). Systemin, a polypeptide signal for proteinase inhibitor gene expression in plants. In: Mechanisms of plant defense responses. Proc. of the 2nd conference of the European Foundation for Plant pathology, Strasbourg-France, August 24-27, 1992. Ed.B. Fritig and M. Legrand. Kluwer academic publishers, pp. 196-201.
C. Abstracts
  1. Chetty, V, Garcia D, Akhari K, Saleh R, and Orozco-Cárdenas ML (2013) Effect of the PARP inhibitor 3-Methoxybenzamide on in vitro plant growth, microtuberization and transformation efficiency of blue potato. In vitro Cellular and Developmental Biology, June 15-19, Providence, Rhode Island – USA
  2. Garcia, DJ, Castillo, F, Ortega, E, Escamilla, E. and Orozco-Cárdenas, ML. (2013). In vitro propagation of Macadamia (Macadamia integrifolia L.). In vitro Cellular and Developmental Biology, June 15-19, Providence, Rhode Island - USA
  3. García, DJ, Alegría, AH, Narváez, J, Orozco-Cárdenas, ML (2012). Genetic Transformation of Soursop (Annona muricata) via Agrobacterium tumefaciens. In vitro Cellular & Dev. Biol. 48(4): 434-435
  4. Ceballos, N., Chetty JV, Lopez, W, Narváez-Vásquez, J, Garcia, D, Orozco-Cárdenas, ML. Evaluation of four Agrobacterium tumefaciens strains for the genetic transformation of Tomato (Solanum lycopersicum L. cultivar Micro-Tom. 2012 World Congress on In vitro Cellular & Cellular Developmental Biology-Seattle, WA. Abstract 15215030.
  5. Garrido-Gutierrez, MI, Cerda, C, Garcia-Rojas, M, Orozco-Cárdenas, ML Ramos Valdivia, AC (2009). Transformación genética de Uncaria tomentosa para la produccción de alcaloides monoterpénicos. XIII Congreso Nacional de Biotecnología y Bioiongeniería. VII Simposium Internacional de producción de alcoholes y levaduras. Acapulco, Guerrero, México.
  6. Mathews, DM, Orozco-Cárdenas, M.L, Giles, JE. and Dodds, JA. (2008).Optimizing tissue culture of verbena for virus exclusion and detection. In: Proceedings of the 12th International Symposium on Virus Diseases of Ornamental Plants, Haarlem, The Netherlands, April 20-28. 65p.
  7. Ngo, X, Lynn, JP, Giles, J, Lovatt, C, Narváez-Vásquez, J, Orozco-Cárdenas, M.L. (2008). Tissue-specific and developmental regulation of Floral genes from citrus. In: Proceedings of the World Congress on In Vitro Biology. Tucson, Arizona. June 14-18.
  8. Narváez-Vásquez, J, Orozco-Cárdenas, ML, Ryan, CA. (2008). Systemic wound signalling in tomato leaves is cooperatively regulated by multiple plant peptides. In: Proceedings of the ASPB Plant biology. Merida, Mexico. June 26-July 1th.
  9. Becerra B, Narváez-Vásquez, J, Orozco-Cárdenas, ML, Lovatt, C. (2008). Regulation of flowering in citrus: preliminary results on the role of water-deficit stress and abscisic acid. 11th International Citrus Congress. Wuhan, China. Oct 26-30.
  10. Garrido-Gutiérrez, MI, Cerda-García-Rojas C.M, Orozco-Cárdenas ML., Ramos-Valdivia AC. (2008). Agrobacterium rhizogenes - mediate transformation of Uncaria tomentosa root cultures for monoterpenoid oxindole alkaloid production. 7th Joint Meeting of AFERP, ASP, GA, PSE and SIF. Natural Products with pharmaceutical, nutraceutical, cosmetic and Agrochemical Interest. Athens, Greece.
  11. Garrido-Gutiérrez, MI., Cerda-García-Rojas CM, Orozco-Cárdenas M.L., Ramos-Valdivia, AC. (2008). Agrobacterium rhizogenes - mediate transformation of Uncaria leaf explants for monoterpenoid alkaloid production. Proceeding ICBB First international Congress on Biotechnology and Bioengineering. Mexico, D.F.
  12. Luna-Palencia GR., Cerda-Garcia-Rojas, CM, Orozco-Cárdenas, ML, Ramos Valdivia AC. (2007). Biosynthesis of oxindole alkaloid in plantlets and roots cultures of Uncaria tomentosa. In: Proceedings of the PSE conference for human health in the post-genome era. Helsinki, Finland. August 26-28.
  13. Ye, Xinrong, Orozco-Cárdenas, ML. (2006). Plant transformation technologies for crop improvement and gene function studies in citrus. In: Procceding of the Centennial Symposium – “Agricultural sustainability and new technologies the next100 years”. Riverside, CA
  14. Port A, Giles, J, Pilliteri, LJ., Lovatt, CJ, Orozco-Cárdenas, ML. (2005) Expression of a Citrus Leafy Gene in Tobacco plants results in early flowering and shoot-flower conversion. In: Proceedings of the America Society of Plant Physiologists. Seattle, WA.
  15. Orozco-Cárdenas, ML. (2005) Hydrogen Peroxide and Nitric Oxide play different roles in plant defense responses. In: Proccedings of the third International Conference Ecological Chemistry, Chisinau, Moldova.
  16. Orozco-Cárdenas, ML, Ryan, CA. (2003). Polygalacturonase ß-subunit antisense gene expression in tomato plants leads to a progressive enhanced wound response and necrosis in leaves and abscission of developing flowers. In: Proceedings of the American Society of Plant Physiologists. Honolulu, Hawaii.
  17. Orozco-Cárdenas, M.L, Ryan, C. A. (2002). Nitric oxide modulates wound signaling in tomato plants. Plant Physiol. In: Proceedings of the American Society of Plant Physiologists. Denver, Colorado.
  18. Orozco-Cárdenas, ML, Narváez-Vásquez, J, Ryan, CA. (2000). Role of hydrogen peroxide as a second messenger in the induction of defense genes in tomato plants in response to wounding, elicitors and methyl jasmonate. In: Proceedings of the American Society of Plant Physiologists. San Diego, California.
  19. Orozco-Cárdenas, ML, Bergey, D, Ryan, CA. (1999). Polygalacturonase activity and hydrogen peroxide are generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway. In: Proceedings of the American Society of Plant Physiologists. Baltimore, Maryland.
  20. Orozco-Cárdenas, ML, Bergey, D, Ryan, CA. (1998). A novel wound- inducible polygalacturonase in tomato plants. In: Phytochemical Society of North America conference: Phytochemicals in human health protection, nutrition and plant defense. Pullman, Washington.
  21. Hernandez, JF, Marino, L, Orozco-Cárdenas, ML, Nárvaez-Vásquez, J. (1995).
  22. Caracterization biologica y molecular de cepas nativas de Bacillus thuringiensis y perspectivas para el mejoramiento. In: Proceedings of the Second Meeting of the Latin-American Network for Plant Biotechnology/REDBIO’95.
  23. Orozco-Cárdenas, ML, Schoofs, H, Swennen, R, Narváez-Vásquez, J. (1995). Celulas embriogenicas en suspension como una alternativa para el mejoramiento de banano. In: Proceedings of the Second Meeting of the Latin-American Network for Plant Biotechnology/REDBIO’95, and The Third National meeting on Plant Biotechnology, Puerto Iguazu-Argentina.
  24. Schaller, A, Pearce, G, Narváez-Vásquez, J, Orozco-Cárdenas, ML, and Ryan, CA. (1994). Systemin: Analyses of early signaling events. In: The 4th international Congress of Plant Molecular Biology, Amsterdam-Netherlands.
  25. Narváez-Vásquez, J, Orozco-Cárdenas, ML, Franceschi, VR. Ryan, CA. (1992). Expression of proteinase inhibitor genes in transgenic plants. In: Sixth International Symposium on Molecular Plant-Microbe Interactions. University of Washington-Seattle, Washington, USA.
D. Theses
  1. Orozco-Cárdenas, ML. (2000). The role of a wound-inducible polygalacturonase and hydrogen peroxidase in the activation of plant defensive genes. Ph.D. thesis, Washington State University, Pullman, WA
  2. Orozco-Cárdenas, ML. (1993). Transformation of tomato plants with an antisense prosystemin gene: Effects on plant resistance towards a Lepidopteran larvae. M.Sc. thesis, Washington State University, Pullman, WA
  3. Orozco-Cárdenas, ML. (1981). Behavior of six varieties of rice (Oryza sativa L.) under different systems of sowing in a saline and sodic soil. BS. Thesis. Universidad Nacional de Colombia, Palmira-Valle, Colombia, SA.


V. Others

A. Patents

1. Ryan, CA, Orozco-Cárdenas, M.L.
International publication number: W02000/063347
PCT Filing number: PCT/US/2000/009465
National entry: Oct. 16, 2001
Title: Active Oxigen species (AOS) to enhance against predator attack.

2. Ryan, CA, Orozco-Cárdenas, ML, McGurl, B., and Pearce, G.
WSURF Case No. 504
Title: “Methods for enhancing plant defense”.
Status: Application No. 09/937,980. Date issued: September 13, 2000

3. Ryan, CA. Orozco-Cárdenas, ML, Bergey, DR.
WSURF Case No. 386.
Title: A tomato Line with Unregulated Natural Defense against Herbivores and
Pathogens".
Status: Application No. 06/325,402. Date issued: June 29, 1998

4. Ryan, CA, Orozco-Cardenas, ML.
Title: “Methods for enhancing plant defense
Canadian Patents database, CIPO Patent # 2370584
PCT filling date: April 6, 2000
PCT publication date: October 26, 2000
PCT filling #: PCT/US2000/009465
International publication number: W02000/063347

B. DNA accessions

1. Chetty, JV, Orozco-Cárdenas, ML (2013). GenBank data base accession No. KF170836. Role of Solanum tuberosum subs. Andigenum (blue potato) in abiotic stress. May 2013

2. Bergey, D.R, Orozco-Cárdenas, ML, De Moura, D and Ryan, CA. GenBank
data base accession No. AF118567. Lycorpersicon esculentum cv.Castlemart. A wound
and systemin-inducible gene encoding polygalacturonase protein in tomato leaves.
February 1999.

 

More Information 

General Campus Information

University of California, Riverside
900 University Ave.
Riverside, CA 92521
Tel: (951) 827-1012

Department Information

Plant Transformation Research Center
South Campus Dr. Head house no. 2
Riverside, CA 92521-0124

Tel: (951) 827-2885
Fax: (951) 827-6325
E-mail: mlorozco@citrus.ucr.edu

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